| 1Levamisole |
|---|
Compound Name Levamisole |
CID 26879 |
Molecular Formula C11H12N2S |
Molecular Weight 204 |
No. Hydrogen Bond Acceptors 2 |
Applications
Linearity curve of Levamisole from O fluid on a Strata-X-C and Knx 2.6um, XB-C!8 50x4.6 column
24533
Separation Mode: Reversed Phase
Reversed Phase
XB-C18
HPLC
Antirheumatic Agents
Applications
Linearity curve of Levamisole from O fluid on a Strata-X-C and Knx 2.6um, XB-C!8 50x4.6 column
Analytes
Details
LC Conditions (App ID: 24533)
Column: | ||||||||||||||||||||||||
Brand Name: Kinetex | ||||||||||||||||||||||||
Part No: | ||||||||||||||||||||||||
Phase Name: XB-C18 | ||||||||||||||||||||||||
Elution Type: Gradient | ||||||||||||||||||||||||
Mobile Phase: A: 0.1% Formic Acid in DI H2O B: 0.1% Formic Acid in ACN | ||||||||||||||||||||||||
Gradient Profile:
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Flow Rate: 1000 µL/min | ||||||||||||||||||||||||
Temperature: 22°C | ||||||||||||||||||||||||
Detection: Tandem Mass Spec (MS-MS) (22°C) | ||||||||||||||||||||||||
Detector Info: Instrument: Agilent® 1260
MS/MS Instrument: SCIEX Triple Quad 5000; ESI Source (+ve ionization)
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Sample Note: Sample pre-treatment
1ml human oral fluid was collected on cellulose pad of the applicator tip provided by the Intercept® i2
oral fluid device (OFC) . Saturated pad was placed into
transport tube containing buffer solution and allowed to
sit overnight. Centrifuge at 600g for 15mins to collect
supernatant.
SPE cartridge: Strata-X-C, 30 mg 96-Well Plate
Part No. 8E-S029-TGB
Step Procedure
Condition: 1 mL Methanol
Equilibrate: 1 mL DI Water
Load: Combine 0.5mL of pretreated sample spiked with internal standards and 1 mL 1% formic acid, mix/vortex 10-15 secs and load in Strata-X-C well plate.
Weak Wash: 1 mL DI Water
Strong Wash: 1 mL 50:50 Acetone/Water
Dry Down: 5 minutes at maximum vacuum (15" Hg or higher)
Elute: 2 x 500 µL Methanol/Acetonitrile/30% Ammonium Hydroxide (5:5:2)
Dry Down: Evaporate to dryness under gentle nitrogen and 45-50°C.
Reconstitute: 200 µL initial mobile phase |
Order Items Used in This Application
00B-4496-E0
Kinetex 2.6 µm XB-C18 100 Å, LC Column 50 x 4.6 mm, Ea
Qty:
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