| 1Metanephrine |
|---|
| 2Normetanephrine |
| 33-Methoxytyramine |
Compound Name Metanephrine |
CID 21100 |
Molecular Formula C10H15NO3 |
Molecular Weight 197 |
No. Chiral Centers: 1 |
No. Hydrogen Bond Acceptors 4 |
No. Hydrogen Bond Donors 3 |
Applications
uCATs by LCMS using Luna Omega 3um Polar C18 50x4.6mm
24171
Separation Mode: Reversed Phase
Reversed Phase
Polar C18
HPLC
Clinical
Clinical Research
Therapeutic / Clinical
Analytes
Details
LC Conditions (App ID: 24171)
Column: | ||||||||||||
Brand Name: | ||||||||||||
Part No: | ||||||||||||
Phase Name: Polar C18 | ||||||||||||
Elution Type: Gradient | ||||||||||||
Mobile Phase: A: Water with 0.1% formic acid B: Methanol with 0.1% formic acid | ||||||||||||
Gradient Profile:
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Flow Rate: 0.7 mL/min | ||||||||||||
Temperature: 25°C | ||||||||||||
Detection: Tandem Mass Spec (MS-MS) (22°C) | ||||||||||||
Detector Info: SCIEX API 4000™ |
Sample Preparation Details
Description: Strata-X-CW Polymeric Weak Cation, microelution 96-well plate, 2mg/well, EA |
Part Number: |
Pre Treatment Note: Pool eluents and dilute with 100 µL of 0.1% Formic acid in water |
Condition 1: Methanol |
Condition 2: 50 mM Ammonium acetate, pH 7 |
Sample PreTreatment: Pool eluents and dilute with 100 µL of 0.1% Formic acid in water |
Wash 1: 50 mM Ammonium acetate, pH 7 |
Wash 2: Acetonitrile / Isopropyl acohol (1:1) |
Evaporation: Evaporate to dryness |
1 Condition Methanol |
2 Shake Water / Acetonitrile / Formic acid (85:10:5) |
1 Shake Water / Acetonitrile / Formic acid (85:10:5) |
2 Shake Water / Acetonitrile / Formic acid (85:10:5) |
1 Shake Water / Acetonitrile / Formic acid (85:10:5) |
2 Shake Water / Acetonitrile / Formic acid (85:10:5) |
Order Items Used in This Application
00B-4760-E0
Luna Omega 3 µm Polar C18 100 Å, LC Column 50 x 4.6 mm, Ea
Qty:
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