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Applications
Water Soluble Vitamins on Kinetex 2.6u Polar C18 100x4.6mm
24038
Separation Mode: Reversed Phase
Reversed Phase
Polar C18
HPLC
Vitamin B Complex
Food & Beverage
Food and Beverage (incl. additives
contaminants)
Product / Trade Good

Applications

Water Soluble Vitamins on Kinetex 2.6u Polar C18 100x4.6mm
Analytes
1Thiamine
2Nicotinamide
3Pyridoxal
4Pyridoxine
5Panthothenic Acid
6Riboflavin
Compound Name
Thiamin
CID
1130
Molecular Formula
C12H17N4OS+
Molecular Weight
265
No. Hydrogen Bond Acceptors
4
No. Hydrogen Bond Donors
2
Total Formal Charge
1
Details
LC Conditions (App ID: 24038)
Column:
Brand Name:
Kinetex
Part No:
Phase Name:
Polar C18
Elution Type:
Gradient
Mobile Phase:
A: 20mM Potassium Phosphate pH 3.5
B: Methanol
Gradient Profile:
Step No.Time(min)%A%B
101000
211000
3104060
4114060
511.11000
6151000
Flow Rate:
1.2 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 210 nm (22°C)

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