| 1C13:0 |
|---|
| 2C16:0 |
| 3C18:0 |
| 4C18:1 cis 9 |
| 5C18:2 trans 9,12 |
| 6C20:0 |
| 7C20:1 cis 11 |
Compound Name Methyl Tridecanoate |
CID 15608 |
Molecular Formula C14H28O2 |
Molecular Weight 228 |
No. Hydrogen Bond Acceptors 2 |
Applications
FAMEs In Peanut Oil by GC/FID on Zebron ZB-FAME
23968
Separation Mode: Application Specific
Application Specific
ZB-FAME
GC
Enzyme Inhibitors
Food & Beverage
Analytes
Details
GC Conditions (App ID: 23968)
Column: |
Part No: |
Phase Name: ZB-FAME |
Injection: Split 100:1 @ 240°C, 1.0 μL |
Oven Program: 180 °C isothermal |
Carrier Gas: Constant Flow Helium, 1.2 mL/min |
Detection: Flame Ionization (FID) (240°C) |
Sample Notes: Recommended Accessories:
Recommended Liner: Zebron PLUS Single Taper with Wool, 4 mm ID
Liner Part No.: AG2-0A11-05 (for Agilent systems)
Inlet Seal: AG0-8620 (Gold Plated Easy Seal)
Septum: AG0-4696 (PhenoRed-400)
Sample Preparation:
1. Weigh 0.2 g of sample into a glass test tube
2. Add 3.8 mL of hexane and vortex
3. Condition SPE tube (Strata® Si-1, 2 g/12 mL, Part No.: 8B-S012-KDG) under vacuum with 6 mL of hexane; do not let bed go dry
4. Load sample with light vacuum
5. Elute with 5 mL of hexane:ethyl acetate (87:13)
6. Evaporate under nitrogen at 35 °C
7. Reconstitute with 4 mL of hexane
8. Add 200 µL of 2 M potassium hydroxide/methanol solution and vortex
9. Leave at room temperature for 5 minutes
10. Add 2 mL of deionized water and vortex
11. Leave to separate until upper layer becomes clear
12. Extract upper layer for GC analysis |
Order Items Used in This Application
7KG-G033-10
Zebron ZB-FAME, GC Cap. Column 60m x 0.25mm x 0.2 µm, Ea
Qty:
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C13:0
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