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50°C,1 min, 25°C/min to 175°C, 4°C/min to 230°C, hold 5 min
Carrier Gas:
Constant Pressure Helium, 33.0 cm/sec
Detection:
Flame Ionization (FID) (280°C)
Sample Notes:
Recommended Accessories:
Recommended Liner: Zebron PLUS Single Taper with Wool, 4 mm ID
Liner Part No.: AG2-0A13-05 (for Agilent systems)
Inlet Seal: AG0-8620 (Gold Plated Easy Seal)
Septum: AG0-4696 (PhenoRed-400)
Sample Preparation:
1. Weigh 100 mg of sample into 20 mL test tube
2. Add 10 mL of hexane
3. Add 100 µL of 2N potassium hydroxide in methanol
4. Vortex for 30 seconds
5. Centrifuge and transfer upper layer into autosampler vial