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100 ºC for 2 min to 140 ºC @ 10 °C/min to 190 ºC @ 3 ºC/min to 260 ºC @ 30ºC/min for 2 min
Carrier Gas:
Constant Flow Helium, 1.2 mL/min
Detection:
Flame Ionization (FID) (260°C)
Sample Notes:
Recommended Liner: Zebron PLUS Single Taper with Wool, 4 mm ID
Liner Part No.: AG2-0A11-05 (for Agilent systems)
Inlet Seal: AG0-8620 (Gold Plated Easy Seal)
Septum: AG0-4696 (PhenoRed-400)
Sample Preparation:
1. Weigh out 100 to 200 mg of peanut butter and place it into a scintillation vial
2. Add 100 mg pyrogallic acid
3. Add 2 mL ethanol followed by 10 mL 8.3 M HCl
4. Incubate at 70 °C for 45 min
5. Extract using 2 mL diethyl ether and 2 mL chloroform
6. Blow down extraction liquid
7. Reconstitute in 1 mL toluene and 2 mL 8 % boron trifluoride in methanol
8. Cap reaction mixture, hold @ 100 °C for 45 min
9. After reaction, add 5 mL water, 1 mL hexane, and 1 g Na2SO4
10. Extract hexane layer for GC analysis