| 1Nornicotine |
|---|
| 23-OH-Cotinine |
| 3Anabasine |
| 4Cotinine |
| 5Nicotine |
Compound Name Nornicotine |
CID 412 |
Molecular Formula C9H12N2 |
Molecular Weight 148 |
No. Chiral Centers: 1 |
No. Hydrogen Bond Acceptors 2 |
No. Hydrogen Bond Donors 1 |
Applications
Cotinine using SPE with Gemini NX-C18, LC-MSMS (Fig.3b)
22037
Separation Mode: Reversed Phase
Reversed Phase
NX-C18
HPLC
Nicotinic Agonists
Analytes
Details
LC Conditions (App ID: 22037)
Column: | ||||||||||||||||||||
Brand Name: | ||||||||||||||||||||
Part No: | ||||||||||||||||||||
Phase Name: NX-C18 | ||||||||||||||||||||
Elution Type: Gradient | ||||||||||||||||||||
Mobile Phase: A: 20mM Ammonium Bicarbonate B: 100% Acetonitrile | ||||||||||||||||||||
Gradient Profile:
| ||||||||||||||||||||
Flow Rate: 500 µL/min | ||||||||||||||||||||
Temperature: 25°C | ||||||||||||||||||||
Detection: Tandem Mass Spec (MS-MS) (22°C) | ||||||||||||||||||||
Detector Info: SCIEX API 4000™ |
Sample Preparation Details
Description: Strata-X-C 33 µm Polymeric Strong Cation, 60 mg / 3 mL, Tubes, 50/Pk |
Part Number: |
Pre Treatment Note: Reconstitution Solvent: 500uL Acetonitrile/20mM Ammonium bicarbonate (10:90) |
Condition 1: Methanol |
Condition 2: Ammonium acetate buffer |
Sample PreTreatment: Reconstitution Solvent: 500uL Acetonitrile/20mM Ammonium bicarbonate (10:90) |
Wash 1: Ammonium acetate buffer |
Wash 2: 30% Methanol |
Evaporation: Evaporate to dryness |
1 Condition Methanol |
2 Wash 30% Methanol |
1 Shake 1.5% Ammonium hydroxide methanol solution |
2 Wash 30% Methanol |
1 Shake 1.5% Ammonium hydroxide methanol solution |
2 Wash 30% Methanol |
Order Items Used in This Application
00B-4453-B0
Gemini 3 µm NX-C18 110 Å, LC Column 50 x 2 mm, Ea
Qty:
Similar Applications
search
Nornicotine
No Data
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.
CONNECT WITH US
