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Applications
Melatonin in commercial formulation on Luna 5u C18(2) 150x4.6mm
20896
Separation Mode: Reversed Phase
Reversed Phase
C18(2)
HPLC
Antioxidants

Applications

Melatonin in commercial formulation on Luna 5u C18(2) 150x4.6mm
Analytes
1Melatonin
Compound Name
Melatonin
CID
896
Molecular Formula
C13H16N2O2
Molecular Weight
232
No. Hydrogen Bond Acceptors
2
No. Hydrogen Bond Donors
2
Details
LC Conditions (App ID: 20896)
Column:
Brand Name:
Part No:
Phase Name:
C18(2)
Elution Type:
Isocratic
Mobile Phase:
A: 0.25% Phosphoric Acid in Water
B: Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
1158020
Flow Rate:
1.5 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 225 nm (22°C)

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