| 1Histamine |
|---|
| 2Tyramine |
Compound Name Histamine |
CID 774 |
Molecular Formula C5H9N3 |
Molecular Weight 111 |
No. Hydrogen Bond Acceptors 2 |
No. Hydrogen Bond Donors 2 |
Applications
OPA Derivitization in Wine of Tyramine and Histamine using Kinetex 2.6u C18
20868
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Sympathomimetics
Food & Beverage
Analytes
Details
LC Conditions (App ID: 20868)
Column: | ||||||||||||||||||||
Brand Name: Kinetex | ||||||||||||||||||||
Part No: | ||||||||||||||||||||
Phase Name: C18 | ||||||||||||||||||||
Elution Type: Gradient | ||||||||||||||||||||
Mobile Phase: A: 20mM Sodium Acetate pH 7.2 w/0.03% Sodium Azide (pre-filtered 0.2um) B: 50:50 Acetonitrile/Methanol | ||||||||||||||||||||
Gradient Profile:
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Flow Rate: 1.2 mL/min | ||||||||||||||||||||
Temperature: 30°C | ||||||||||||||||||||
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 338 nm (22°C) | ||||||||||||||||||||
Detector Info: Offline Amino Acid derivatization:
1. In to a amber vial dispense 250uL of 20mM Sodium Acetate pH 12.5
2. Add 100uL of Sample (ex: Wine sample, Standards)
3. Mix for 30 seconds
4. Add 50uL of OPA derivatization agent (OPA: Part # 5061-3335)
5. Mix vigorously for 30 seconds
6. Add 2.0mL of MilliQ Water
7. Mix for 30 seconds
8. Inject 10uL of sample on to system
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Order Items Used in This Application
00D-4462-E0
Kinetex 2.6 µm C18 100 Å, LC Column 100 x 4.6 mm, Ea
Qty:
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