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Applications
Preparative loading on Kinetex 5u C18 in Axia 50x21.2mm
20774
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Antidepressive Agents, Tricyclic

Applications

Preparative loading on Kinetex 5u C18 in Axia 50x21.2mm
Analytes
1Doxepin
2Amitriptyline
Compound Name
Doxepin
CID
667477
Molecular Formula
C19H21NO
Molecular Weight
279
No. Hydrogen Bond Acceptors
2
Details
LC Conditions (App ID: 20774)
Column:
Brand Name:
Part No:
Phase Name:
C18
Elution Type:
Gradient
Mobile Phase:
A: Water with 0.5% Formic Acid
B: Acetonitrile with 0.5% Formic acid
Gradient Profile:
Step No.Time(min)%A%B
108020
285050
3115050
Flow Rate:
30 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (22°C)

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