| 1Metronidazole |
|---|
| 213-C2 15-N-Metronidazole |
Compound Name Metronidazole |
CID 4173 |
Molecular Formula C6H9N3O3 |
Molecular Weight 171 |
No. Hydrogen Bond Acceptors 4 |
No. Hydrogen Bond Donors 1 |
Applications
Extraction and Analysis of Metronidazole in Facial Cleansers
20631
Separation Mode: Reversed Phase
Reversed Phase
XB-C18
HPLC
Antiprotozoal Agents
Personal Care/Consumer Products
Personal Healthcare / Cosmetic
Product / Trade Good
Analytes
Details
LC Conditions (App ID: 20631)
Column: | ||||||||||||||||||||||||
Brand Name: Kinetex | ||||||||||||||||||||||||
Part No: | ||||||||||||||||||||||||
Phase Name: XB-C18 | ||||||||||||||||||||||||
Elution Type: Gradient | ||||||||||||||||||||||||
Mobile Phase: A: 0.1% Formic Acid B: MeOH | ||||||||||||||||||||||||
Gradient Profile:
| ||||||||||||||||||||||||
Flow Rate: 300 µL/min | ||||||||||||||||||||||||
Temperature: 25°C | ||||||||||||||||||||||||
Detection: Mass Spectrometer (MS) (650°C) | ||||||||||||||||||||||||
Detector Info: SCIEX API 4000™ | ||||||||||||||||||||||||
Sample Note: SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C18 for 2.1mm ID Columns, 3/Pk Part No.: AJ0-8782
- Holder Part No.: AJ0-9000 |
Sample Preparation Details
Description: Strata-X-C 33 µm Polymeric Strong Cation, 30 mg / 3 mL, Tubes, 50/Pk |
Part Number: |
Pre Treatment Note: Evaporate to dryness at 50 C. Reconstitute in 200 uL of 10:90 MeOH:0.1% Formic Acid |
Condition 1: 1 mL of MeOH |
Condition 2: 1 mL of 0.1N HCl |
Sample PreTreatment: Evaporate to dryness at 50 C. Reconstitute in 200 uL of 10:90 MeOH:0.1% Formic Acid |
Wash 1: 3 mL of 0.1N HCl |
Wash 2: 3 mL of MeOH |
Wash 3: 6 mL of Ethyl Acetate |
Evaporation: Evaporate to dryness |
1 Condition 1 mL of MeOH |
2 Wash 3 mL of MeOH |
1 Condition 1 mL of MeOH |
1 Shake 1 mL of 5:95 NH4OH:MeOH (v:v) |
2 Condition 1 mL of 0.1N HCl |
3 Wash 6 mL of Ethyl Acetate |
Order Items Used in This Application
00B-4496-AN
Kinetex 2.6 µm XB-C18 100 Å, LC Column 50 x 2.1 mm, Ea
Qty:
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