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Applications
Water soluble vitamins on Kinetex 2.6µm C18, 100x4.6mm
20596
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Vitamin B Complex
Food & Beverage
Biochemical Compound / Nutrient
Therapeutic / Clinical
Product / Trade Good
Therapeutic / Clinical

Applications

Water soluble vitamins on Kinetex 2.6µm C18, 100x4.6mm
Analytes
1Thiamine
2Ascorbic acid
3Niacinamide
4p-Aminobenzoic acid
5Vitamin B12
6Folic acid
Compound Name
Thiamin
CID
1130
Molecular Formula
C12H17N4OS+
Molecular Weight
265
No. Hydrogen Bond Acceptors
4
No. Hydrogen Bond Donors
2
Total Formal Charge
1
Details
LC Conditions (App ID: 20596)
Column:
Brand Name:
Kinetex
Part No:
Phase Name:
C18
Elution Type:
Gradient
Mobile Phase:
A: 20mM Phosphate buffer pH 3
B: Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
10955
2107525
Flow Rate:
1 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 214 nm (22°C)
Sample Note:
SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime. - SecurityGuard ULTRA Cartridges, UHPLC C18 for 4.6mm ID Columns, 3/Pk Part No.: AJ0-8768 - Holder Part No.: AJ0-9000

Order Items Used in This Application

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