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Applications
Penicillins in Meat by LC/MS/MS
20028
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Anti-Bacterial Agents

Applications

Penicillins in Meat by LC/MS/MS
Analytes
1Amoxicillin
2Ampicillin
3Penicillin G
4Penicillin V
5Oxacillin
6Cloxacillin
7Nafcillin
8Dicloxacillin
Compound Name
Amoxicillin
CID
33613
Molecular Formula
C16H19N3O5S
Molecular Weight
365
No. Hydrogen Bond Acceptors
6
No. Hydrogen Bond Donors
4
Details
LC Conditions (App ID: 20028)
Column:
Brand Name:
Part No:
Phase Name:
C18
Elution Type:
Gradient
Mobile Phase:
A: 0.1% Ammonia in water
B: 95% Acetronitrile, 5% water and 0.1% ammonia
Gradient Profile:
Step No.Time(min)%A%B
10982
22982
32.429010
465050
56.10100
66.60100
76.8982
88982
Flow Rate:
0.8 mL/min
Temperature:
40°C
Detection:
Tandem Mass Spec (MS-MS) @ -4500 V (600°C)
Detector Info:
3200 QTRAP
Sample Note:
This separation uses IMethod Kit Part Number KH0-9047 SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Gemini C18 4 x 3.0mm ID, 10/Pk Part No.: AJ0-7597 - Holder Part No.: KJ0-4282
Sample Preparation Details
Description:
Strata C18-E (55 µm, 70 Å), 200 mg / 3 mL, Tubes, 50/Pk
Part Number:
Pre Treatment Note:
The equilibration time at conditon 0 was 4 minutes before each injection.
Condition 1:
Methanol
Condition 2:
Water
Sample PreTreatment:
The equilibration time at conditon 0 was 4 minutes before each injection.
Wash 1:
0.1% Formic Acid
Evaporation:
Evaporate to dryness
1
Condition
Methanol
2
Shake
Acetonitrile
1
Shake
Acetonitrile
2
Shake
Acetonitrile
1
Shake
Acetonitrile

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