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Applications
Chloramphenicol in Seafood and dairy products by LC/MS/MS
20026
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Anti-Bacterial Agents
Food & Beverage
Pharmaceutical
Therapeutic / Clinical

Applications

Chloramphenicol in Seafood and dairy products by LC/MS/MS
Analytes
1Chloramphenicol
2Chloramphenicol
3Chloramphenicol-d5
Compound Name
Chloramphenicol
CID
5959
Molecular Formula
C11H12Cl2N2O5
Molecular Weight
323
No. Hydrogen Bond Acceptors
5
No. Hydrogen Bond Donors
3
Details
LC Conditions (App ID: 20026)
Column:
Brand Name:
Kinetex
Part No:
Phase Name:
C18
Elution Type:
Gradient
Mobile Phase:
A: Water
B: Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
1-2955
20955
32955
42.01595
54.5595
Flow Rate:
0.4 mL/min
Temperature:
20°C
Detection:
Tandem Mass Spec (MS-MS) (450°C)
Detector Info:
SCIEX API 4000™
Sample Note:
SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime. - SecurityGuard ULTRA Cartridges, UHPLC C18 for 2.1mm ID Columns, 3/Pk Part No.: AJ0-8782 - Holder Part No.: AJ0-9000
Sample Preparation Details
Description:
Strata-X 33 µm Polymeric Reversed Phase, 60 mg / 3 mL, Tubes, 50/Pk
Part Number:
Condition 1:
Methanol
Condition 2:
Water
Wash 1:
Water
Evaporation:
Evaporate to dryness
1
Condition
Methanol
2
Condition
Water
1
Shake
Ethyl Acetate
1
Condition
Methanol

Order Items Used in This Application

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