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Applications
Water-soluble Vitamins on Kinetex 2.6u, C18, 150x4.6 by HPLC
20000
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Vitamin B Complex
Food & Beverage
Therapeutic / Clinical
Product / Trade Good
Biochemical Compound / Nutrient
Product / Trade Good

Applications

Water-soluble Vitamins on Kinetex 2.6u, C18, 150x4.6 by HPLC
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Analytes
1Ascorbic acid
2Pyridoxal 5'-phosphate
3Nicotinic acid
44-Pyridoxic acid
5Nicotinamide
6Riboflavin 5'-phosphate
7Pyridoxal
8Pyridoxine
9Cobalamine
10Folic acid
11Pyridoxamine
12Riboflavin
13Thiamine
Compound Name
Ascorbic Acid
CID
5785
Molecular Formula
C6H8O6
Molecular Weight
176
No. Hydrogen Bond Acceptors
6
No. Hydrogen Bond Donors
4
Details
LC Conditions (App ID: 20000)
Column:
Brand Name:
Kinetex
Part No:
Phase Name:
C18
Elution Type:
Gradient
Mobile Phase:
A: 3g Sodium 1-hexanesulfonate monohydrate/0.4mL Triethylamine/15mL Acetic acid in 1985mL H2O
B: Methanol
Gradient Profile:
Step No.Time(min)%A%B
10955
2125347
312.1298
415298
515.1955
622955
Flow Rate:
0.5 mL/min
Temperature:
25°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 270 nm (22°C)

Order Items Used in This Application

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