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A: Water with 0.1% TFA B: Acetonitrile with 0.1% TFA
Gradient Profile:
Step No.
Time(min)
%A
%B
1
0
97
3
2
3
97
3
3
23
35
65
Flow Rate:
0.312 mL/min
Temperature:
90°C
Detection:
UV-Vis Abs.-Diode Array (PDA) @ 214 nm (22°C)
Sample Note:
Application was actually run on a 50x4.6mm ID running at a flow rate of 1.5 mL/min. 50 x 4.6 mm which is not a commercially available part number. Information switched to 50x2.1mm for those that wish to replicate method.