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Applications
25-OH Vitamin D2 and D3 Standards from Serum on Kinetex 2.6µm C18 after PPT
19313
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Vitamins
Clinical

Applications

25-OH Vitamin D2 and D3 Standards from Serum on Kinetex 2.6µm C18 after PPT
Analytes
125-Hydroxy vitamin D3 (25-OH D3)
225-Hydroxy vitamin D3-d6 (25-OH D3-d6)
325-Hydroxy vitamin D2 (25-OH D2)
Compound Name
Calcifediol
CID
5283731
Molecular Formula
C27H44O2
Molecular Weight
400
No. Hydrogen Bond Acceptors
2
No. Hydrogen Bond Donors
2
Details
LC Conditions (App ID: 19313)
Column:
Brand Name:
Kinetex
Part No:
Phase Name:
C18
Elution Type:
Gradient
Mobile Phase:
A: 0.05 % Formic acid
B: 5 mM Ammonium acetate + 0.1 % Formic acid in methanol
Gradient Profile:
Step No.Time(min)%A%B
10928
25928
32050100
42900100
5360928
Flow Rate:
1 mL/min
Temperature:
0°C
Detection:
Mass Spectrometer (MS) (450°C)
Detector Info:
SCIEX API 4000™

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Kinetex 2.6 µm C18 100 Å, LC Column 50 x 4.6 mm, Ea
Reversed Phase