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Applications
USP 621: Allowable changes Column Diameter-4.6mm ID
19228
Separation Mode: Reversed Phase
Reversed Phase
C18(2)
HPLC
Antihypertensive Agents
Pharmaceutical: Therapeutic Uses
Therapeutic / Clinical

Applications

USP 621: Allowable changes Column Diameter-4.6mm ID
Analytes
1Atenolol
2Impurity B
3Impurity F
4Impurity G
5Impurity I
6Impurity J
Compound Name
Atenolol
CID
2249
Molecular Formula
C14H22N2O3
Molecular Weight
266
No. Chiral Centers:
1
No. Hydrogen Bond Acceptors
4
No. Hydrogen Bond Donors
3
Details
LC Conditions (App ID: 19228)
Column:
Brand Name:
Part No:
Phase Name:
C18(2)
Elution Type:
Isocratic
Mobile Phase:
A: See analyst note
Gradient Profile:
Step No.Time(min)%A
10100
Flow Rate:
0.8 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) (22°C)
Sample Note:
Isocratic: 100% of 2.0g of Sodium Octanesulphonate + 0.8g of Tetrabutyl Ammonium Hydrogen Sulphate in 2L of 360ml Methanol + 40ml of THF+1600ml of 3.4g of potassium Dihydrogen Phosphate in 2L of water (pH adjusted to 3.0 using Phosphoric acid)

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