For security purposes

FOR SECURITY PURPOSES - because Internet Explorer is no longer supported by Microsoft, we suggest that you interact with our secure site through one of our supported browsers - Google Chrome, Firefox, or MS Edge. If you continue to use this website with Internet Explorer you do so at your own risk and you may encounter problems.

Applications

Protein Calibration Curve on BioSep-SEC-S4000(2)

Analytes

1Proteins

Analyte not found

Details

LC Conditions (App ID: 18935)

Column:

BioSep 5 µm SEC-s4000 500 Å, LC Column 300 x 7.8 mm, Ea

Brand Name:

Part No:

Phase Name:

SEC-s4000

Elution Type:

Isocratic

Mobile Phase:

A: 100mM Sodium Phosphate, 300mM NaCl, pH 7.0

Gradient Profile:

Step No.	Time(min)	%A
1	0	100

Flow Rate:

1 mL/min

Temperature:

22°C

Detection:

UV-Vis Abs.-Variable Wave.(UV) @ 280 nm (22°C)

Detector Info:

3rd order of polynomial MW calibration curves

Sample Note:

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, GFC 4000 4 x 3.0mm ID, 10/Pk Part No.: AJ0-4489 - Holder Part No.: KJ0-4282

Order Items Used in This Application

00H-2147-K0

BioSep 5 µm SEC-s4000 500 Å, LC Column 300 x 7.8 mm, Ea

Qty:

infoSign in to view country specific pricing

Similar Applications

Select Analyte of Interest

Low MW proteins and peptides on BioSep2000(2) non-denaturing condition

BioSep 5 µm SEC-s2000 145 Å, LC Column 300 x 7.8 mm, Ea

Gel Filtration Chromatography (GFC)

Low MW proteins and peptides on BioSep2000(2)

BioSep 5 µm SEC-s2000 145 Å, LC Column 300 x 7.8 mm, Ea

Gel Filtration Chromatography (GFC)

MW Calibration Proteins on BioSep-SEC-S4000

BioSep 5 µm SEC-s4000 500 Å, LC Column 300 x 7.8 mm, Ea

Gel Filtration Chromatography (GFC)

Protein Calibration Curve on BioSep-SEC-S3000(2)

BioSep 5 µm SEC-s3000 290Å, LC Column 300 x 7.8 mm, Ea

Gel Filtration Chromatography (GFC)

Protein Calibration Curve on BioSep-SEC-S2000(2)

BioSep 5 µm SEC-s2000 145 Å, LC Column 300 x 7.8 mm, Ea

Gel Filtration Chromatography (GFC)

MW Calibration Proteins on BioSep-SEC-S3000(2)

BioSep 5 µm SEC-s3000 290Å, LC Column 300 x 7.8 mm, Ea

Gel Filtration Chromatography (GFC)

Protein Calibration Curves on BioSep-SEC-S (2)

BioSep 5 µm SEC-s3000 290Å, LC Column 300 x 7.8 mm, Ea

Gel Filtration Chromatography (GFC)

PEGylated proteins on Jupiter® 300 C18 3µm and 5µm, and C4 5µm columns

Jupiter 3 µm C18 300 Å, LC Column 150 x 4.6 mm, Ea

Reversed Phase

Overlay of PEGylated vs. Native Proteins on Jupiter® 300 C18 Column

Jupiter 5 µm C18 300 Å, LC Column 150 x 4.6 mm, Ea

Reversed Phase

Overlay of PEGylated vs. Native Proteins on Jupiter® 300 C4 Column

Jupiter 5 µm C4 300 Å, LC Column 150 x 4.6 mm, Ea

Reversed Phase

Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.

CONNECT WITH US

PRODUCTS AND SERVICES

SUPPORT

Product/Method Support

Product Troubleshooting

OUR COMPANY

About Phenomenex

Danaher Life Sciences