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Applications
Water-soluble vitamins on Kinetex 2.6um HILIC 150 x 4.6mm
18842
Separation Mode: Hydrophilic Interaction
Hydrophilic Interaction
HILIC
HPLC
Vitamin B Complex
Food & Beverage
Pharmaceutical
Therapeutic / Clinical

Applications

Water-soluble vitamins on Kinetex 2.6um HILIC 150 x 4.6mm
Analytes
1Nicotinamide
2PABA
3Pyridoxine
4Riboflavin
5Nicotinic acid
6Ascorbic acid
7Folic acid
8Vitamin B12
9Thiamine
Compound Name
Nicotinamide
CID
936
Molecular Formula
C6H6N2O
Molecular Weight
122
No. Hydrogen Bond Acceptors
2
No. Hydrogen Bond Donors
1
Details
LC Conditions (App ID: 18842)
Column:
Brand Name:
Kinetex
Part No:
Phase Name:
HILIC
Elution Type:
Gradient
Mobile Phase:
A: Acetonitrile
B: 100mM Ammonium formate pH 3.2
C: Water
Gradient Profile:
Step No.Time(min)%A%B%C
1090100
2590100
320501040
Flow Rate:
1 mL/min
Temperature:
0°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 260 nm (22°C)

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