Applications

Selectivity Comparison for Steroids on Kinetex 2.6µm C18 150 x 4.6mm ID

Analytes

111-alpha-Hydroxyprogesterone
2Cortisone acetate
3Estradiol
421-Hydroxyprogesterone
511-Ketoprogesterone
6Estrone

Compound Name 11Alpha-Hydroxyprogesterone
CID 92730
Molecular Formula C21H30O3
Molecular Weight 330
No. Hydrogen Bond Acceptors 3
No. Hydrogen Bond Donors 1

Details

LC Conditions (App ID: 18684)

Column:

Kinetex 2.6 µm C18 100 Å, LC Column 150 x 4.6 mm, Ea

Brand Name:

Kinetex

Part No:

00F-4462-E0

Phase Name:

C18

Elution Type:

Gradient

Mobile Phase:

A: Water

B: ACN

Gradient Profile:

Step No.	Time(min)	%A	%B
1	0	75	25
2	12	35	65
3	12.1	75	25
4	16	75	25

Flow Rate:

1.2 mL/min

Temperature:

0°C

Detection:

UV-Vis Abs.-Diode Array (PDA) @ 230 nm (30°C)

Order Items Used in This Application

00F-4462-E0

Kinetex 2.6 µm C18 100 Å, LC Column 150 x 4.6 mm, Ea

Qty:

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