FOR SECURITY PURPOSES - because Internet Explorer is no longer supported by Microsoft, we suggest that you interact with our secure site through one of our supported browsers - Google Chrome, Firefox, or MS Edge. If you continue to use this website with Internet Explorer you do so at your own risk and you may encounter problems.
Different ratios of HFIP to TEA were investigated using the poly dT 12-18 standard mixture. HFIP was fixed at 200 mM and different levels of TEA were used between 1 and 20 mM (4mm-red trace {pH 7.6}, 8mm-blue trace{pH8.0} and 14mm -black trace{pH8.3}). Optimal retention, MS response, and resolution were observed for the 200 mM HFIP./ 8mM TEA mobile phase mixture.