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Applications
Erythromycin on Gemini-NX 5 um C18 250 x 4.6
18085
Separation Mode: Reversed Phase
Reversed Phase
NX-C18
HPLC
Pharmaceutical
Therapeutic / Clinical

Applications

Erythromycin on Gemini-NX 5 um C18 250 x 4.6
Analytes
1Erythromycin F (EF)
2Erythromycin C (EC)
3Erythromycin A (EA)
4Anhydroerythromycin A (AEA)
5Erythromycin B (EB)
6Pseudo-erythromycin A enol ether
7Erythromycin A enol ether (EAEN)
Compound Name
Erythromycin F
CID
6426644
Molecular Formula
C37H67NO14
Molecular Weight
749
No. Chiral Centers:
10
No. Hydrogen Bond Acceptors
15
No. Hydrogen Bond Donors
6
Details
LC Conditions (App ID: 18085)
Column:
Brand Name:
Part No:
Phase Name:
NX-C18
Elution Type:
Isocratic
Mobile Phase:
A: Ammonium Carbonate (10mM) in H2O
B: Tertiary Butyl Alcohol
C: Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B%C
1080155
Flow Rate:
0.8 mL/min
Temperature:
55°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 215 nm (22°C)
Sample Note:
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Gemini-NX C18 4 x 3.0mm, 10/Pk Part No.: AJ0-8368 - Holder Part No.: KJ0-4282

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