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Applications
Plasma Metanephrines by LC/MS/MS on Luna HILIC following SPE with Strata-X-CW
17970
Clinical
Clinical Research
Therapeutic / Clinical

Applications

Plasma Metanephrines by LC/MS/MS on Luna HILIC following SPE with Strata-X-CW
Analytes
1Metanephrine
2Metanephrine-d3
3Normetanephrine
4Normetanephrine-d3
Compound Name
Metanephrine
CID
21100
Molecular Formula
C10H15NO3
Molecular Weight
197
No. Chiral Centers:
1
No. Hydrogen Bond Acceptors
4
No. Hydrogen Bond Donors
3
Details
LC Conditions (App ID: 17970)
Elution Type:
Gradient
Mobile Phase:
A: ACN/100mM Ammonium Formate, pH 3.2 (95:5)
B: ACN/Water/100mM Ammonium Formate, pH 3.2 (50:45:5)
Gradient Profile:
Step No.Time(min)%A%B
101000
22.510100
33.011000
461000
Flow Rate:
0.4 mL/min
Temperature:
22°C
Detection:
Mass Spectrometer (MS) (22°C)
Detector Info:
Analyte MRM Pair (Q1/Q3) MN 180.4/148.2 MN-D3 183.4/151.2 NMN 166.4/134.2 NMN-D3 169.4/137.2
Sample Note:
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, HILIC 4 x 2.0mm, 10/Pk Part No.: AJ0-8328 - Holder Part No.: KJ0-4282
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