| 1PEGylated Proteins |
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Analyte not found
Applications
PEGylated proteins on Jupiter® 300 C18 3µm and 5µm, and C4 5µm columns
17521
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Pharmaceutical/Biopharmaceutical
Life Science
Protein
Biochemical Compound / Nutrient
Analytes
Details
LC Conditions (App ID: 17521)
Column: | ||||||||||||
Brand Name: | ||||||||||||
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Phase Name: C18 | ||||||||||||
Elution Type: Gradient | ||||||||||||
Mobile Phase: A: 0.1% TFA, 2% ACN in Water B: 90% ACN, 0.085% TFA in Water | ||||||||||||
Gradient Profile:
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Flow Rate: 1 mL/min | ||||||||||||
Temperature: 45°C | ||||||||||||
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 214 nm (25°C) | ||||||||||||
Sample Note: Compare reverse phase separations of different PEGylated and intact proteins selected at different reaction time-points on three different columns. (A) PEGylated alpha-Chymotrypsinogen A with PEG1 Amine 20kDa Methoxy PEG NHS-ester, 0 min fraction; (B) PEGylated alpha-Chymotrypsinogen A with PEG3 N-Terminus 20kDa Methoxy PEG aldehyde, 120 min fraction; (C) PEGylated beta-Lactoglobulin A with PEG4 N-Terminus 40kDa Y-shape Methoxy PEG aldehyde, overnight fraction. Well-noticed, good resolution was achieved on Jupiter 3µm C18 small particles versus larger Jupiter 5µm C18 particles or Jupiter C4 bonded media. |
Order Items Used in This Application
00F-4263-E0
Jupiter 3 µm C18 300 Å, LC Column 150 x 4.6 mm, Ea
Qty:
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