For security purposes
FOR SECURITY PURPOSES - because Internet Explorer is no longer supported by Microsoft, we suggest that you interact with our secure site through one of our supported browsers - Google Chrome, Firefox, or MS Edge. If you continue to use this website with Internet Explorer you do so at your own risk and you may encounter problems.
Applications
Nicotine in Ammonium Bicarbonate pH 10.5 on Gemini-NX 5µ
16627
Separation Mode: Reversed Phase
Reversed Phase
NX-C18
HPLC
Nicotinic Agonists
Clinical
Pharmaceutical
Therapeutic / Clinical

Applications

Nicotine in Ammonium Bicarbonate pH 10.5 on Gemini-NX 5µ
Analytes
1Nicotine
Compound Name
Nicotine
CID
89594
Molecular Formula
C10H14N2
Molecular Weight
162
No. Hydrogen Bond Acceptors
2
Details
LC Conditions (App ID: 16627)
Column:
Brand Name:
Part No:
Phase Name:
NX-C18
Elution Type:
Gradient
Mobile Phase:
A: 10 mM Ammonium Bicarbonate pH 10.5
B: Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
10955
210595
312595
Flow Rate:
1.5 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (22°C)
Sample Note:
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4287 - Holder Part No.: KJ0-4282

Order Items Used in This Application

Similar Applications
search
Kinetex 2.6 µm EVO C18 100 Å, LC Column 100 x 3.0 mm, EA
Reversed Phase
Kinetex 5 µm EVO C18 100 Å, LC Column 100 x 3.0 mm, Ea
Reversed Phase
Synergi 4 µm Max-RP 80 Å, LC Column 150 x 4.6 mm, Ea
Reversed Phase
Gemini 3 µm NX-C18 110 Å, LC Column 100 x 2 mm, Ea
Reversed Phase
Gemini 3 µm NX-C18 110 Å, LC Column 50 x 2 mm, Ea
Reversed Phase
Gemini 5 µm NX-C18 110 Å, LC Column 150 x 4.6 mm, Ea
Reversed Phase
Gemini 5 µm C18 110 Å, LC Column 100 x 2 mm, Ea
Reversed Phase
Luna 5 µm HILIC 200 Å, LC Column 100 x 2 mm, Ea
Hydrophilic Interaction
Luna 3 µm Phenyl-Hexyl 100 Å, LC Column 50 x 4.6 mm, Ea
Reversed Phase