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Applications

Steroid Mix (4) on Luna PFP(2)

Analytes

1Prednisone
2Prednisolone
3Hydroxyprogesterone
4Progesterone

Compound Name Prednisone
CID 5865
Molecular Formula C21H26O5
Molecular Weight 358
No. Hydrogen Bond Acceptors 5
No. Hydrogen Bond Donors 2

Details

LC Conditions (App ID: 16288)

Column:

Luna 5 µm PFP(2) 100 Å, LC Column 150 x 4.6 mm, Ea

Brand Name:

Part No:

Phase Name:

PFP(2)

Elution Type:

Gradient

Mobile Phase:

A: Water

B: Acetonitrile

Gradient Profile:

Step No.	Time(min)	%A	%B
1	0	60	40
2	8	30	70
3	10	30	70

Flow Rate:

1 mL/min

Temperature:

22°C

Detection:

UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (22°C)

Detector Info:

Rs(1,2) = 2.84

Sample Note:

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, PFP 4 x 3.0 mm, 10/Pk Part No.: AJ0-8327 - Holder Part No.: KJ0-4282

Order Items Used in This Application

00F-4448-E0

Luna 5 µm PFP(2) 100 Å, LC Column 150 x 4.6 mm, Ea

Qty:

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