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A: 0.1% TFA and 2% ACN in Water B: 70/20% ACN/IPA, 0.08% TFA in Water
Gradient Profile:
Step No.
Time(min)
%A
%B
1
0
85
15
2
25
30
70
Flow Rate:
1 mL/min
Temperature:
45°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 214 nm (22°C)
Sample Note:
Reversed phase of PEGylated Insulin run on listed columns. Note the reduced resolution for the Jupiter C18 and EXP 2 columns when compared to the Jupiter C4 column. Although it is difficult to visualize, the Jupiter actully has better resolution of PEGylated forms than the EXP1 column.