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Applications
Tricyclic Antidepressants at Neutral pH by Gemini 5um C18
16131
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Antidepressive Agents, Tricyclic
Pharmaceutical
Therapeutic / Clinical

Applications

Tricyclic Antidepressants at Neutral pH by Gemini 5um C18
Analytes
1Imipramine
2Nortriptyline
3Amitriptyline
4Clomipramine
Compound Name
Imipramine
CID
3696
Molecular Formula
C19H24N2
Molecular Weight
280
No. Hydrogen Bond Acceptors
2
Details
LC Conditions (App ID: 16131)
Column:
Brand Name:
Part No:
Phase Name:
C18
Elution Type:
Isocratic
Mobile Phase:
A: 20mM Potassium Phosphate pH7.0
B: Acetontrile
C: Methanol
Gradient Profile:
Step No.Time(min)%A%B%C
10303535
Flow Rate:
1.5 mL/min
Temperature:
25°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (25°C)
Sample Note:
*** Results are the average of 6 injections Peak 3, N=9032, SYMM=0.85 Peak 4, N=9190, SYMM=0.89 Average of 4 peaks, N=8498 Column Back Pressure = 117bar

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