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Applications
Thymine family using Luna 5u C18(2) (acetate)
15760
Separation Mode: Reversed Phase
Reversed Phase
C18(2)
HPLC
Biochemical - NOT Polymeric (incl. oligomers)
Biochemical Compound / Nutrient

Applications

Thymine family using Luna 5u C18(2) (acetate)
Analytes
1TTP
2Thymidine 5'-pyrophosphate
3TMP
4Thymine
5Thymidine
Compound Name
Phosphoric Acid
CID
1004
Molecular Formula
H3O4P
Molecular Weight
97
No. Hydrogen Bond Acceptors
4
No. Hydrogen Bond Donors
3
Details
LC Conditions (App ID: 15760)
Column:
Brand Name:
Part No:
Phase Name:
C18(2)
Elution Type:
Gradient
Mobile Phase:
A: 20mM Ammonium acetate, pH 4.5
B: Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
10973
2106040
Flow Rate:
1 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 260 nm (22°C)
Sample Note:
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4287 - Holder Part No.: KJ0-4282

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