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Applications

Steroids on Onyx C18

Analytes

1Prednisolone
2Cortisone
3Betamethasone
4Corticosterone
511-Hydroxyprogesterone
6Estradiol
711-Ketoprogesterone
8Estrone
9Progesterone

Compound Name Prednisolone
CID 5755
Molecular Formula C21H28O5
Molecular Weight 360
No. Hydrogen Bond Acceptors 5
No. Hydrogen Bond Donors 3

Details

LC Conditions (App ID: 15509)

Column:

Onyx Monolithic C18, LC Column 100 x 4.6 mm, Ea

Brand Name:

Part No:

Phase Name:

Monolithic C18

Elution Type:

Gradient

Mobile Phase:

A: Water

B: Acetonitrile

Gradient Profile:

Step No.	Time(min)	%A	%B
1	0	80	20
2	7	10	90

Flow Rate:

3 mL/min

Temperature:

22°C

System:

Agilent (HP) 1100

Detection:

UV-Vis Abs.-Variable Wave.(UV) @ 220 nm (22°C)

Order Items Used in This Application

CH0-7643

Onyx Monolithic C18, LC Column 100 x 4.6 mm, Ea

Qty:

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