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Applications
Caffeine & Metabolites, pH 10.0
15012
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Phosphodiesterase Inhibitors
Food & Beverage
Food and Beverage (incl. additives
contaminants)
Product / Trade Good

Applications

Caffeine & Metabolites, pH 10.0
Analytes
1Xanthine
2Theobromine
3Theophylline
4Caffeine
Compound Name
Xanthine
CID
1188
Molecular Formula
C5H4N4O2
Molecular Weight
152
No. Hydrogen Bond Acceptors
3
No. Hydrogen Bond Donors
3
Details
LC Conditions (App ID: 15012)
Column:
Brand Name:
Part No:
Phase Name:
C18
Elution Type:
Gradient
Mobile Phase:
A: 20mM NH4HCO3, pH 10.0
B: Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
18982
278020
Flow Rate:
1 mL/min
Temperature:
25°C
System:
Agilent (HP) 1100
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 273 nm (25°C)
Sample Note:
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Gemini C18 4 x 3.0mm, 10/Pk Part No.: AJ0-7597 - Holder Part No.: KJ0-4282

Order Items Used in This Application

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