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A: 60 mL 0.20M (NH4)2HPO4 (pH 6.5), 60 mL 0.20M tetrabutyl (NH4)HSO4 (pH6.5), 100 mL ACN, dilute to 1L with H2O B: 60 mL 0.20M (NH4)2HPO4 (pH 6.5), 60 mL 0.20M tetrabutyl (NH4)HSO4 (pH6.5), 500 mL ACN, dilute to 1L with H2O
Gradient Profile:
Step No.
Time(min)
%A
%B
1
0
78
22
2
39
26
74
3
47
26
74
Flow Rate:
1.3 mL/min
Temperature:
45°C
Detection:
UV-Vis Abs.-Fixed Wave. (UV) @ 205 nm (45°C)
Sample Note:
SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4287
- Holder Part No.: KJ0-4282