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Applications
Sennosides
12959
Separation Mode: Reversed Phase
Reversed Phase
Phenyl-Hexyl
HPLC
Cathartics
Food & Beverage
Nutraceutical
Biochemical Compound / Nutrient

Applications

Sennosides
Analytes
1Sennoside B
2Sennoside A
Compound Name
Sennoside
CID
5199
Molecular Formula
C42H38O20
Molecular Weight
862
No. Chiral Centers:
12
No. Hydrogen Bond Acceptors
20
No. Hydrogen Bond Donors
12
Details
LC Conditions (App ID: 12959)
Column:
Brand Name:
Part No:
Phase Name:
Phenyl-Hexyl
Elution Type:
Gradient
Mobile Phase:
A: 0.1% TFA in water
B: 0.1% TFA in Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
108416
2458020
3481090
4541090
5558416
Flow Rate:
1 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 270.4 nm (22°C)
Sample Note:
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Phenyl 4 x 3.0mm, 10/Pk Part No.: AJ0-4351 - Holder Part No.: KJ0-4282

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