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Applications
Nicotine and Cotinine in Human Plasma
1278
Separation Mode: Reversed Phase
Reversed Phase
Phenyl-Hexyl
HPLC
Indicators and Reagents
Clinical
Pharmaceutical
Therapeutic / Clinical

Applications

Nicotine and Cotinine in Human Plasma
Analytes
1Nicotine
2Cotinine
Compound Name
Nicotine
CID
89594
Molecular Formula
C10H14N2
Molecular Weight
162
No. Hydrogen Bond Acceptors
2
Details
LC Conditions (App ID: 1278)
Column:
Brand Name:
Part No:
Phase Name:
Phenyl-Hexyl
Elution Type:
Isocratic
Mobile Phase:
A: Methanol
B: 10mM Ammoniom Acetate
Gradient Profile:
Step No.Time(min)%A%B
105050
Flow Rate:
1 mL/min
Temperature:
22°C
Detection:
Mass Spectrometer (MS) (22°C)
Sample Note:
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Phenyl 4 x 3.0mm, 10/Pk Part No.: AJ0-4351 - Holder Part No.: KJ0-4282

Order Items Used in This Application

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Hydrophilic Interaction