Available in over 40 unique achiral and chiral selectivities, Axia advanced preparative column packings and column hardware designs offer several advantages. Unlike traditional preparative HPLC column packing methods, the Axia packing method offers increased sorbent bed density for increased performance and eliminates media bed collapse as a source of premature column failure in preparative HPLC/SFC columns.
Axia packed preparative columns involve a single axial compression step, unlike conventional packed preparative columns. The ideal prep HPLC column bed density is custom calculated and automated for each specific media and column size. Computer control of the entire process ensures both proper bed density and column uniformity every time.
The highly tuned patented process and hardware eliminates potential decompression ensuring bed stability and optimal packing density. The media found on the inlet frit of the Axia packed column shows no signs of damage unlike the media found on inlet frit of traditionally packed HPLC prep columns.
Decompression and then recompression during packing can damage the media and lead to increased column-to-column variability, flow disturbances, and decreased column lifetimes.
The automated Axiaâ„¢ packing system provides feedback control and infinite tuning of packing density for specific media characteristics such as mechanical strength and porosity. An optimum higher bed density can be consistently reproduced column-to-column. This directly translates into consistent efficiency and peak asymmetry measurements and decreases the column variability seen in traditionally packed preparative columns.
Columns:
Waters® XTerra® 5 µm Prep MS C18 OBD™
|
Dimension:
50 x 21.2 mm (Luna)
50 x 19 mm (XTerra) |
Mobile Phase:
A: 0.5 % TFA in Water
B: 0.5 % TFA in Acetonitrile Formic Acid |
Gradient:
Linear 95:5 (A/B) to 5:95 (A/B)
over 7 min, hold 3 min |
Flow Rate:
30 mL/min (Luna)
24 mL/min (XTerra)‡ |
Temperature:
Ambient
|
Injection Volume:
500 μL
|
Detection:
UV @ 254 nm
|
Sample:
1. Triprolidine 1.6 mg
2. Methacycline 16 mg 3. Amitriptyline 5.25 mg |
Gradient:
10 min on 100 mm
length column
25 min on 250 mm
length column
5 % to 95 % Acetonitrile,
0.5 % TFA
|
Flow Rate:
30 mL/min
|
Dimension:
50 x 4.6 mm
|
Sample Load:
0.1 mg in 21 µL DMSO
|
Flow Rate
1.5 mL/min |
Dimension:
50 x 21.2 mm
|
Sample Load:
2.1 mg in 420 µL DMSO
|
Flow Rate
30 mL/min |
Dimension:
50 x 30 mm
|
Sample Load:
4.2 mg in 840 µL DMSO
|
Flow Rate
60 mL/min |
Dimension:
50 x 50 mm
|
Sample Load:
10.5 mg in 2100 µL DMSO
|
Flow Rate
150 mL/min |
Column:
|
Dimension:
150 x 4.6 mm
|
Mobile Phase:
Hexane / Ethanol (75:25)
|
Flow Rate:
1 mL/min
|
Temperature:
Ambient
|
Injection Volume:
100 µL
|
Column:
|
Dimension:
150 x 21.2 mm
|
Mobile Phase:
Hexane / Ethanol (75:25)
|
Flow Rate:
20 mL/min
|
Temperature:
Ambient
|
Injection Volume:
20 mL
|
Column (mm) |
Analytical 150 x 4.6 |
Standard 150 x 21.2 |
Axia 150 x 21.2 |
---|---|---|---|
Mass Loaded (mg) | 2 | 40 | 40 |
Resolution* | 1.5 | 2.85 | 3.72 |
Plates (N) | 117 | 535 | 760 |
Axia column and packing technology is patented by Phenomenex. U.S. Patent No. 7, 674, 383