Kinetex Core-Shell Technology delivers dramatic improvements in efficiency over conventional fully porous media, which can be leveraged to increase resolution, significantly improve productivity, reduce solvent consumption, and decrease costs. Whether you are running high-performance liquid chromatography (HPLC) or ultra-high performance liquid chromatography (UHPLC) methods, the Kinetex core-shell family can deliver shockingly improved performance over the current column you are using. Phenomenex designs, manufactures, and sells its very own silica and organo-silica core-shell particles.
Core-shell technology in chromatography is a revolutionary approach that enhances the efficiency of HPLC systems while minimizing costs. Core-shell particles are designed with a solid, non-porous silica core surrounded by a porous outer layer. This unique structure allows for faster analyte mass transfer between the mobile and stationary phases, as diffusion occurs only through the thin, porous shell rather than the entire particle. This minimizes band broadening, leading to higher separation efficiency and sharper peaks.
The consistency in size and shape of core-shell particles further enhances separation efficiency by reducing variability in analyte movement. As a result, core-shell HPLC columns provide high efficiency and resolution at lower backpressures, making them an ideal choice for labs looking to improve their existing HPLC systems without the need for costly UHPLC equipment.
Core-shell technology thus offers a cost-effective alternative to upgrading to UHPLC systems, enabling labs to maximize the efficiency of their current HPLC setups. The formation of these particles through advanced sol-gel processing techniques ensures uniformity and reliability, making them a valuable tool in modern chromatography. By using the Kinetex HPLC column, laboratories can achieve faster analysis times and higher productivity while maintaining excellent separation performance.
Using sol-gel processing techniques that incorporate nano-structuring technology, a durable, homogeneous porous shell is grown on a solid silica core to create a Kinetex Core-Shell particle. This particle morphology results in less band broadening for all four sources of UHPLC band broadening compared to fully porous particles and thus delivers extremely high efficiencies.
Minimized Band Broadening
Increased Band Broadening
Kinetex Phases
|
Shipping Solvent†
|
Particle
Sizes (µm) |
Pore
Size (Å) |
Surface
Area (m2/g) |
Carbon
Load (%) |
pH Stability
|
Reversed
Phase |
Normal
Phase |
HILIC
|
100%
Aqueous Stable |
---|---|---|---|---|---|---|---|---|---|---|
Acetonitrile / Water (50:50)
|
2.6
|
100
|
200
|
9
|
1.5-8.5*
|
|
||||
Acetonitrile / Water (50:50)
|
2.6
|
100
|
200
|
9
|
1.5-8.5*
|
|
||||
Acetonitrile / Water (50:50)
|
1.3, 1.7, 2.6, 5
|
100
|
200
|
12
|
1.5-8.5*
|
|
||||
Acetonitrile / Water (45:55)
|
1.7, 2.6, 5
|
100
|
200
|
11
|
1-12
|
|
|
|||
Acetonitrile / Water (50:50)
|
1.7, 2.6, 3.5, 5
|
100
|
200
|
10
|
1.5-8.5*
|
|
||||
Acetonitrile / Water (45:55)
|
1.7, 2.6, 5
|
100
|
200
|
8
|
1.5-8.5*
|
|
||||
Acetonitrile/Water w/
0.1 % Formic Acid (50:50) |
1.7, 2.6, 5
|
100
|
200
|
11
|
1.5-8.5*
|
|
|
|||
Acetonitrile / Water (45:55)
|
1.7, 2.6, 5
|
100
|
200
|
11
|
1.5-8.5*
|
|
||||
Acetonitrile / Water (40:60)
|
1.7, 2.6, 5
|
100
|
200
|
9
|
1.5-8.5*
|
|
|
|
||
Acetonitrile /
100 mM Ammonium Formate (93:7) |
1.7, 2.6, 5
|
100
|
200
|
0
|
2.0-7.5
|
|
|
|||
Acetonitrile / Water (65:35)
|
3.5
|
—
|
—
|
12
|
1.5-8.5*
|
|
The combination of a consistent, solid, high-density core along with proprietary column packing technologies in the Kinetex HPLC column ensures optimum bed structure and high column performance. This carefully designed core structure promotes uniform packing of the particles, which is essential for minimizing band-broadening effects that can arise from Eddy Diffusion.
The manufacturing process of Kinetex columns is tightly controlled, focusing on factors such as particle size distribution, surface and bonding homogeneity, and packing quality. This meticulous engineering leads to uniform packing and minimized band-broadening, resulting in superior separation efficiency.
Columns:
Kinetex 2.6 µm C18
Traditional 1.7 µm C18 |
Dimension:
50 x 2.1 mm
|
Mobile Phase:
Acetonitrile / Water (50:50)
|
Flow Rate:
0.6 mL/min
|
Temperature:
25 °C
|
Detection:
UV @ 254 nm
|
Instrument:
Waters® ACQUITY® UPLC®
|
Sample:
0.5 µL test mixture
1. Acetophenone
2. Benzene
3. Toluene
4. Naphthalene
|
Every step in the manufacturing process of Kinetex Core-Shell columns is tightly controlled for:
Columns:
Kinetex 2.6 µm C18
|
Dimension:
50 x 4.6 mm
|
Part No.:
00B-4462-E0
|
Mobile Phase:
Water / Acetonitrile (65:35)
|
Flow Rate:
1.0 mL/min
|
Temperature:
25 °C
|
Detection:
UV @ 254 nm
|
Sample:
1. Uracil
2. Hydrocortisone
3. Cortisone
4. Cortisone acetate
5. 17-Hydroxyprogesterone
|