Phenogel size exclusion columns for gel permeation chromatography (GPC) are composed of highly cross-linked styrene-divinylbenzene (SDVB) and achieve high resolution and tight linear calibration curves. Phenogel GPC columns are available in seven different pore sizes, ranging from 50 Ã… to 106 Ã…; and a linear bed configuration. Pore size distribution and pore volume are closely controlled parameters in the manufacturing process, accounting for high resolution, tight linear calibration curves, and excellent column-to-column reproducibility. USP L21 packings available.
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Phenogel packing materials are made from styrene-divinylbenzene by emulsion polymerization under conditions to optimize pore size, total pore volume, particle size, and degree of cross-linking. The results yield Gel Permeation Chromatography (GPC) materials that provide very high resolution, rugged durability, and wide solvent compatibility.
Recommended GPC Solvent | Sample | Suggested Temp. |
---|---|---|
THF
|
Polystyrenes |
Ambient
|
Polybutadienes | – | |
Epoxy Resins | – | |
Phenolic Resins | Ambient | |
Polymethyl Methacrylates | – | |
Polyethylene Glycol | – | |
HFIP*
|
Polyamides (Nylon) |
30 °C
|
PET/PETP | – | |
Dichloromethane
|
Naphthalene |
–
|
Diethylhexyl Phthalate | Ambient | |
Toluene
|
Polyisobutylene |
Ambient
|
Polyisoprene | – | |
Silicone Oils | – | |
DMF
|
Polyethylene Oxide |
50 °C
|
Polyvinylpyrrolidone (PVP) | 40 °C | |
Cellulose Acetate | – | |
Hydroxyethylcellulose (HEC) | – | |
Chlorinated Rubber | – |
*HFIP (hexafluoroisopropanol) allows polymers such as polyamides and PET that are analyzed at a temperature of 135 °C and higher to be analyzed at temperatures below 100 °C. The narrow particle distribution of Phenogel columns eliminates the problem of overlapping peaks and band broadening that is typically associated with using this solvent with traditional GPC columns.
Phenogel Pore Size: | |||||||||
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Mobile Phase Solvent | 50 Ã… | 100 Ã… | 500 Ã… | 103 Ã… | 104 Ã… | 105 Ã… | 106 Ã… | Linear & Mixed |
Suggested Operating Temp. |
Acetone | Y | Y | Y | Y | Y | Y | Y | Y | |
Benzene | Y | Y | Y | Y | Y | Y | Y | Y | |
Carbon Tetrachloride | Y | Y | Y | Y | Y | Y | Y | Y | |
Chloroform | Y | Y | Y | Y | Y | Y | Y | Y | |
30 % HFIP/Chloroform | Y | Y | Y | Y | Y | Y | Y | Y | |
Diethyl Ether | Y | Y | Y | Y | Y | Y | Y | Y | |
Dimethylacetamide (DMAC) | Y* | Y | Y | Y | Y | Y | Y | Y | 60 °C |
Dimethylformamide (DMF) | Y* | Y | Y | Y | Y | Y | Y | Y | 60 °C |
Dioxane | Y | Y | Y | Y | Y | Y | Y | Y | |
DMSO | Y* | Y | Y | Y | Y | Y | Y | Y | 60 °C |
Ethyl Acetate | Y | Y | Y | Y | Y | Y | Y | Y | |
Hexafluoroisopropanol (HFIP) | Y | Y | Y | Y | Y | Y | Y | Y | |
Hexane | Y | Y | Y | Y | Y | Y | Y | Y | |
M-Cresol | Y* | Y | Y | Y | Y | Y | Y | Y | 100 °C |
Methyl Ethyl Ketone | Y | Y | Y | Y | Y | Y | Y | Y | |
Methylene Chloride | Y | Y | Y | Y | Y | Y | Y | Y | |
0-Chlorophenol | Y* | Y | Y | Y | Y | Y | Y | Y | 100 °C |
0-Dichlorobenzene | Y* | Y | Y | Y | Y | Y | Y | Y | 135 °C |
Quinolin | Y* | Y | Y | Y | Y | Y | Y | Y | 60 °C |
Tetrahydrofuran | Y | Y | Y | Y | Y | Y | Y | Y | |
Toluene | Y | Y | Y | Y | Y | Y | Y | Y | |
Trichlorobenzene | Y* | Y | Y | Y | Y | Y | Y | Y | 135 °C |
Water | N | N | N | N | N | N | N | N | |
Xylene | Y | Y | Y | Y | Y | Y | Y | Y |
*Not recommended on 5 µm 50 Å columns.
N = Not Compatible
Y = Compatible